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versadoc mp 4000 imaging system  (Bio-Rad)


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    Structured Review

    Bio-Rad versadoc mp 4000 imaging system
    Versadoc Mp 4000 Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/versadoc+4000+imager/ChemiDoc+MP+Imaging+System/pmc11610704__CBIC___25___e202400441___s001-224-9-19
    Average 99 stars, based on 22840 article reviews
    versadoc mp 4000 imaging system - by Bioz Stars, 2026-09
    99/100 stars

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    Software:

    Article Title: cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1
    Article Snippet: Blots were probed with an anti-DGKθ (1:1000, HPA026797; Sigma-Aldrich, St. Louis, MO), anti-SREBP1 (1:1000, sc-8984; Santa Cruz Biotechnology, Santa Cruz, CA), SF1 (1:4000; Millipore, Temecula, CA), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10,000, sc-25778; Santa Cruz Biotechnology) and an anti-rabbit or mouse secondary antibody (1:5000, ECF Western blotting reagent; GE Healthcare, Piscataway, NJ). .. Blots were scanned on a VersaDoc 4000 Imager (Bio-Rad, Hercules, CA) and densitometric analysis carried out using Quantity One software (Bio-Rad). .. Nuclear and cytoplasmic extract isolation H295R cells were cultured in 100 mm dishes and treated with Bt 2 cAMP for 48 h. Cytoplasmic and nuclear extracts were harvested from H295R cells and separated using Thermo NE-PER ® nuclear and cytoplasmic extraction reagents (Pierce Biotechnology, Rockford, IL).

    Article Title: cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1
    Article Snippet: Blots were probed with an anti-DGK (1:1000, HPA026797; SigmaAldrich, St. Louis, MO), anti-SREBP1 (1:1000, sc-8984; Santa Cruz Biotechnology, Santa Cruz, CA), SF1 (1:4000; Millipore, Temecula, CA), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10,000, sc-25778; Santa Cruz Biotechnology) and an anti-rabbit or mouse secondary antibody (1:5000, ECF Western blotting reagent; GE Healthcare, Piscataway, NJ). .. Blots were scanned on a VersaDoc 4000 Imager (Bio-Rad, Hercules, CA) and densitometric analysis carried out using Quantity One software (Bio-Rad). .. Nuclear and cytoplasmic extract isolation H295R cells were cultured in 100 mm dishes and treated with Bt 2 cAMP for 48 h. Cytoplasmic and nuclear extracts were harvested from H295R cells and separated using Thermo NE-PER ® nuclear and cytoplasmic extraction reagents (Pierce Biotechnology, Rockford, IL).

    other:

    Article Title: Oxysterol-Related-Binding-Protein Related Protein-2 (ORP2) Regulates Cortisol Biosynthesis and Cholesterol Homeostasis
    Article Snippet: Briefly, Ultra Performance Liquid Chromatography (UPLC) tandem Mass Spectroscopy (MS-MS) was carried out with a Waters Acquity UPLC system connected with the Xevo TQ triple quadrupole mass spectrometer ( Gaikwad NW, 2013 ).

    Staining:

    Article Title: Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation
    Article Snippet: .. Sypro Ruby staining was detected with a Bio-Rad VersaDoc 4000 imager (excitation 300nm, 520nm longpass emission). ..

    Article Title: Metabolic Labeling of Caenorhabditis elegans Primary Embryonic Cells with Azido-Sugars as a Tool for Glycoprotein Discovery
    Article Snippet: After electrophoresis, TAMRA fluorescence was recorded with a Typhoon Trio (GE) (ex 532 nm; em 580 nm BP). .. Gels were then stained with Sypro Ruby (Invitrogen), and imaged with a Typhoon Trio (ex 488 nm, em 610 nm BP) or Bio-Rad VersaDoc 4000 imager (ex 300 nm, em 520 nm LP). .. Gels were analyzed using Bio-Rad PDQuest 8.0, and spots excised using Bio-Rad ExQuest Spot Cutter.

    Expressing:

    Article Title: cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1
    Article Snippet: Output blots were probed with anti-SF1 (1:5000, 07-618; Millipore) and input blots with anti-FLAG (1:2500, F1804, Sigma). .. Expression was detected using an ECF Western blotting kit (GE Biosciences) and visualized using a VersaDoc 4000 imager (Bio-Rad). ..

    Article Title: p54 nrb /NONO Regulates Cyclic AMP-Dependent Glucocorticoid Production by Modulating Phosphodiesterase mRNA Splicing and Degradation
    Article Snippet: Aliquots of each sample (30 μg of protein) were resolved on 8% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Temecula, CA). .. Blots were probed with the antibodies listed in , and expression was detected using an ECF Western blotting reagent kit (GE Healthcare, Piscataway, NJ) and visualized by scanning the blots on a VersaDoc 4000 imager (Bio-Rad, Hercules, CA). .. Protein amounts were determined using the BCA protein assay (Pierce, Rockford, IL). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Target protein Source (catalog no., vendor) p54 nrb /NONO 05-950, Millipore MC2R sc-13107, Santa Cruz MRAP ab103319, Abcam P450scc (CYP11A1) Donated by Michael R. Waterman, Vanderbilt University P450c11B (CYP11B1/2) sc-28205, Santa Cruz P450c17 (CYP17A1) sc-66849, Santa Cruz P450c21B (CYP21A2) sc-134566, Santa Cruz STAR sc-28205, Santa Cruz SF-1 07-618, Millipore NUR77 SAB2101650, Sigma-Aldrich NURR1 N6538, Sigma-Aldrich NOR1 sc-100906, Santa Cruz XRN2 A301-103A, Bethyl PDE11A ab154273, Abcam GAPDH sc-25778, Santa Cruz Open in a separate window List of antibodies RNA isolation and real-time RT-PCR.

    Article Title: p54nrb/NONO Regulates Cyclic AMP-Dependent Glucocorticoid Production by Modulating Phosphodiesterase mRNA Splicing and Degradation
    Article Snippet: Aliquots of each sample (30 g of protein) 177 were resolved on 8% SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) 178 membranes (Millipore, Temecula, CA). .. Blots were probed with antibodies listed in Table 2 and 179 expression was detected using an ECF western blotting reagent kit (GE Healthcare, Piscataway, 180 NJ) and visualized by scanning the blots on a VersaDoc 4000 imager (Bio-Rad, Hercules, CA). .. 181 Protein amounts were determined using the BCA Protein Assay (Pierce, Rockford, IL).

    Western Blot:

    Article Title: cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1
    Article Snippet: Output blots were probed with anti-SF1 (1:5000, 07-618; Millipore) and input blots with anti-FLAG (1:2500, F1804, Sigma). .. Expression was detected using an ECF Western blotting kit (GE Biosciences) and visualized using a VersaDoc 4000 imager (Bio-Rad). ..

    Article Title: p54 nrb /NONO Regulates Cyclic AMP-Dependent Glucocorticoid Production by Modulating Phosphodiesterase mRNA Splicing and Degradation
    Article Snippet: Aliquots of each sample (30 μg of protein) were resolved on 8% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Temecula, CA). .. Blots were probed with the antibodies listed in , and expression was detected using an ECF Western blotting reagent kit (GE Healthcare, Piscataway, NJ) and visualized by scanning the blots on a VersaDoc 4000 imager (Bio-Rad, Hercules, CA). .. Protein amounts were determined using the BCA protein assay (Pierce, Rockford, IL). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Target protein Source (catalog no., vendor) p54 nrb /NONO 05-950, Millipore MC2R sc-13107, Santa Cruz MRAP ab103319, Abcam P450scc (CYP11A1) Donated by Michael R. Waterman, Vanderbilt University P450c11B (CYP11B1/2) sc-28205, Santa Cruz P450c17 (CYP17A1) sc-66849, Santa Cruz P450c21B (CYP21A2) sc-134566, Santa Cruz STAR sc-28205, Santa Cruz SF-1 07-618, Millipore NUR77 SAB2101650, Sigma-Aldrich NURR1 N6538, Sigma-Aldrich NOR1 sc-100906, Santa Cruz XRN2 A301-103A, Bethyl PDE11A ab154273, Abcam GAPDH sc-25778, Santa Cruz Open in a separate window List of antibodies RNA isolation and real-time RT-PCR.

    Article Title: p54nrb/NONO Regulates Cyclic AMP-Dependent Glucocorticoid Production by Modulating Phosphodiesterase mRNA Splicing and Degradation
    Article Snippet: Aliquots of each sample (30 g of protein) 177 were resolved on 8% SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) 178 membranes (Millipore, Temecula, CA). .. Blots were probed with antibodies listed in Table 2 and 179 expression was detected using an ECF western blotting reagent kit (GE Healthcare, Piscataway, 180 NJ) and visualized by scanning the blots on a VersaDoc 4000 imager (Bio-Rad, Hercules, CA). .. 181 Protein amounts were determined using the BCA Protein Assay (Pierce, Rockford, IL).



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